Recombinant Human Butyrylcholinesterase As a New-Age Bioscavenger Drug: Development of the Expression System

نویسندگان

  • D.G. Ilyushin
  • O.M. Haertley
  • T.V. Bobik
  • O.G. Shamborant
  • E.A. Surina
  • V.D. Knorre
  • P. Masson
  • I.V. Smirnov
  • A.G. Gabibov
  • N.A. Ponomarenko
چکیده

Butyrylcholinesterase (BChE) is a serine hydrolase (EC 3.1.1.8) which can be found in most animal tissues. This enzyme has a broad spectrum of efficacy against organophosphorus compounds, which makes it a prime candidate for the role of stoichiometric bioscavenger. Development of a new-age DNA-encoded bioscavenger is a vival task. Several transgenic expression systems of human BChE were developed over the past 20 years; however, none of them has been shown to make economic sense or has been approved for administration to humans. In this study, a CHO-based expression system was redesigned, resulting in a significant increase in the production level of functional recombinant human butyrylcholinesterase as compared to the hitherto existing systems. The recombinant enzyme was characterized with Elman and ELISA methods.

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عنوان ژورنال:

دوره 5  شماره 

صفحات  -

تاریخ انتشار 2013